Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury.
Chin Med J (Engl)
; 124(11): 1708-13, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21740782
ABSTRACT
BACKGROUND:
It remains almost a helpless situation for the recurrent implantation failure and pregnancy loss caused by endometrial injury at present. The purpose of this study was to develop a rabbit model of endometrial mechanical injury that could provide a research platform for this difficult clinical predicament.METHODS:
Three experiments were conducted. Experiment 1 Curettages in both uterus horns and copper wire inserting after curettage (double-injury) in one horn. The histological changes were monitored at 0, 24, 48, 72 hours, as well as in 1 and 2 weeks after operation. Experiment 2 Direct copper wire inserting in one horn and double-injury in other horn. The wires in both horns were removed after 2 weeks. The histological changes were recorded at 0, 1 and 2 weeks after wire removal. Experiment 3 Double-injury procedure in one horn was performed and wire was removed after 2 weeks; another horn was remained normal to serve as control. Histological changes were recorded, tissue areas were measured, and proliferation indices (PIs, %) were calculated at 1, 2, 4 and 8 weeks after wire removal, respectively.RESULTS:
The experiments revealed that the injured endometrium by simple curettage or copper wire could be fully repaired. While the endometrial regeneration was severely impaired by double-injury, both areas of endometrium and uterine cavity decreased (P < 0.05); both PIs of glandular epithelial and stromal cells increased and reached maximum at 4 weeks (P < 0.05), but returned by 8 weeks.CONCLUSION:
This study demonstrated that a rabbit model of endometrial injury could be effectively established through a double-injury procedure of curettage and copper wire with comparable clinical index.
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Bases de dados:
MEDLINE
Assunto principal:
Cobre
/
Curetagem
/
Modelos Animais de Doenças
/
Endométrio
Limite:
Animals
Idioma:
En
Revista:
Chin Med J (Engl)
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China