Sequence homologies between Mycoplasma and Chlamydia spp. lead to false-positive results in chlamydial cell cultures tested for mycoplasma contamination with a commercial PCR assay.
J Clin Microbiol
; 49(10): 3681-2, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21849688
ABSTRACT
Mycoplasma contamination is a frequent problem in chlamydial cell culture. After obtaining contradictory contamination results, we compared three commercial PCR kits for mycoplasma detection. One kit signaled contamination in mycoplasma-free Chlamydia pneumoniae cultures. Sequencing of cloned PCR products revealed primer homology with the chlamydial genome as the basis of this false-positive result.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Infecções por Chlamydia
/
Chlamydia
/
Reação em Cadeia da Polimerase
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Técnicas Bacteriológicas
/
Reações Falso-Positivas
/
Mycoplasma
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Áustria