IL-10 contributes to the suppressive function of tumour-associated myeloid cells and enhances myeloid cell accumulation in tumours.
Scand J Immunol
; 75(3): 273-81, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22050574
ABSTRACT
Studies have revealed that tumour-associated myeloid cells (TAMC) are one of the major sources of IL-10 in tumour-bearing mice. However, the significance of TAMC-derived IL-10 in tumour immunity is poorly understood. Here, we show that IL-10 blockade or IL-10 deficiency reduces the capacity of TAMC in suppressing the proliferation of P1A-specific CD8 T cells. In the spleen, IL-10-deficient and wild-type (WT) mice bearing large tumour burdens have similar TAMC populations. The tumours from IL-10-deficient mice, however, have reduced numbers of TAMC compared with tumours from their WT counterparts. IL-10â»/â» RAG-2â»/â» mice also had reduced numbers of TAMC compared with tumours from IL-10âº/⺠RAG-2â»/â» mice; therefore, the reduction in TAMC in IL-10-deficient tumours was not because of adaptive immune response in tumours. Adoptively transferred tumour antigen-specific CD8 T cells expanded more efficiently within tumours in IL-10â»/â» RAG-2â»/â» mice than in tumours from IL-10âº/⺠RAG-2â»/â» mice. Cytotoxic T lymphocyte adoptive transfer therapy prevented tumour evasion in IL-10â»/â» RAG-2â»/â» mice more efficiently than in IL-10âº/⺠RAG-2â»/â» mice. Thus, IL-10 enhances the accumulation of myeloid cells in tumours, and TAMC-derived IL-10 suppresses the activation and expansion of tumour antigen-specific T cells.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Linfócitos T Citotóxicos
/
Interleucina-10
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Células Mieloides
/
Neoplasias Experimentais
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Scand J Immunol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos