The effects of proton radiation on the prothrombin and partial thromboplastin times of irradiated ferrets.
Int J Radiat Biol
; 88(4): 327-34, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22221163
ABSTRACT
PURPOSE:
To determine whether proton radiation affects coagulation. MATERIAL ANDMETHODS:
Ferrets were exposed to solar particle event-like proton radiation at doses of 0, 25, 100, or 200 centigray (cGy), and dose rates of 50 cGy/minute (high dose rate or HDR) or 50 cGy/hour (low dose rate or LDR). Plasma was isolated from blood collected prior to radiation exposure and at 3-7 h post-radiation. Prothrombin time (PT) assays and activated partial thromboplastin time (aPTT) assays were performed as were mixing studies to determine the coagulation factors involved.RESULTS:
HDR and LDR exposure led to statistically significant increases in PT values. It was determined that the HDR-induced increase in PT was due to Factor VII, while Factors II, V, and VII contributed to the LDR-induced increase in PT values. Only acute LDR exposure caused an increase in aPTT values, which remained elevated for 48 h post-irradiation (which was the latest time assayed in these studies). Mixing studies revealed that Factor IX contributed to the increased aPTT values. A majority of the animals exposed at the LDR had an International Normalized Ratio approaching or surpassing 2.0.CONCLUSIONS:
PT/aPTT assays resulted in increased clotting times due to different coagulation factors, indicating potential radiation-induced coagulopathy.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Prótons
/
Coagulação Sanguínea
/
Protrombina
/
Tromboplastina
/
Furões
Limite:
Animals
Idioma:
En
Revista:
Int J Radiat Biol
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos