High-throughput ballistic injection nanorheology to measure cell mechanics.
Nat Protoc
; 7(1): 155-70, 2012 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-22222790
ABSTRACT
High-throughput ballistic injection nanorheology is a method for the quantitative study of cell mechanics. Cell mechanics are measured by ballistic injection of submicron particles into the cytoplasm of living cells and tracking the spontaneous displacement of the particles at high spatial resolution. The trajectories of the cytoplasm-embedded particles are transformed into mean-squared displacements, which are subsequently transformed into frequency-dependent viscoelastic moduli and time-dependent creep compliance of the cytoplasm. This method allows for the study of a wide range of cellular conditions, including cells inside a 3D matrix, cell subjected to shear flows and biochemical stimuli, and cells in a live animal. Ballistic injection lasts <1 min and is followed by overnight incubation. Multiple particle tracking for one cell lasts <1 min. Forty cells can be examined in <1 h.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Reologia
/
Caenorhabditis elegans
/
Nanotecnologia
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Protoc
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos