An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome.
Proc Natl Acad Sci U S A
; 109(4): 1275-80, 2012 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-22232685
ABSTRACT
The rhs genes are a family of enigmatic composite genes, widespread among Gram-negative bacteria. In this study, we characterized rhsT, a Pseudomonas aeruginosa rhs gene that encodes a toxic protein. Expression of rhsT was induced upon contact with phagocytic cells. The RhsT protein was exposed on the bacterial surface and translocated into phagocytic cells; these cells subsequently underwent inflammasome-mediated death. Moreover, RhsT enhanced host secretion of the potent proinflammatory cytokines IL-1ß and IL-18 in an inflammasome-dependent manner. In a mouse model of acute pneumonia, infection with a P. aeruginosa strain lacking rhsT was associated with less IL-18 production, fewer recruited leukocytes, reduced pulmonary bacterial load, and enhanced animal survival. Thus, rhsT encodes a virulence determinant that activates the inflammasome.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Pneumonia
/
Pseudomonas aeruginosa
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Toxinas Bacterianas
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Fatores de Virulência
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Inflamassomos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos