Blood-borne macrophage-neural cell interactions hitchhike on endosome networks for cell-based nanozyme brain delivery.
Nanomedicine (Lond)
; 7(6): 815-33, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22236307
ABSTRACT
BACKGROUND:
Macrophage-carried nanoformulated catalase ('nanozyme') attenuates neuroinflammation and protects nigrostriatal neurons from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication. This is facilitated by effective enzyme transfer from blood-borne macrophages to adjacent endothelial cells and neurons leading to the decomposition of reactive oxygen species. MATERIALS &METHODS:
We examined the intra- and inter-cellular trafficking mechanisms of nanozymes by confocal microscopy. Improved neuronal survival mediated by nanozyme-loaded macrophages was demonstrated by fluorescence-activated cell sorting.RESULTS:
In macrophages, nanozymes were internalized mainly by clathrin-mediated endocytosis then trafficked to recycling endosomes. The enzyme is subsequently released in exosomes facilitated by bridging conduits. Nanozyme transfer from macrophages to adjacent cells by endocytosis-independent mechanisms diffusing broadly throughout the recipient cells. In contrast, macrophage-free nanozymes were localized in lysosomes following endocytic entry.CONCLUSION:
Facilitated transfer of nanozyme from cell to cell can improve neuroprotection against oxidative stress commonly seen during neurodegenerative disease processes.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Endossomos
/
Encéfalo
/
Catalase
/
Sistemas de Liberação de Medicamentos
/
Nanopartículas
/
Macrófagos
/
Neurônios
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Nanomedicine (Lond)
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos