Maternal xNorrin, a canonical Wnt signaling agonist and TGF-ß antagonist, controls early neuroectoderm specification in Xenopus.
PLoS Biol
; 10(3): e1001286, 2012.
Article
em En
| MEDLINE
| ID: mdl-22448144
Dorsal-ventral specification in the amphibian embryo is controlled by ß-catenin, whose activation in all dorsal cells is dependent on maternal Wnt11. However, it remains unknown whether other maternally secreted factors contribute to ß-catenin activation in the dorsal ectoderm. Here, we show that maternal Xenopus Norrin (xNorrin) promotes anterior neural tissue formation in ventralized embryos. Conversely, when xNorrin function is inhibited, early canonical Wnt signaling in the dorsal ectoderm and the early expression of the zygotic neural inducers Chordin, Noggin, and Xnr3 are severely suppressed, causing the loss of anterior structures. In addition, xNorrin potently inhibits BMP- and Nodal/Activin-related functions through direct binding to the ligands. Moreover, a subset of Norrin mutants identified in humans with Norrie disease retain Wnt activation but show defective inhibition of Nodal/Activin-related signaling in mesoderm induction, suggesting that this disinhibition causes Norrie disease. Thus, xNorrin is an unusual molecule that acts on two major signaling pathways, Wnt and TGF-ß, in opposite ways and is essential for early neuroectoderm specification.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Xenopus
/
Fator de Crescimento Transformador beta
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Proteínas de Xenopus
/
Placa Neural
/
Via de Sinalização Wnt
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
PLoS Biol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China