Development and application of a novel histological multichrome technique for clam histopathology.
J Invertebr Pathol
; 110(3): 411-4, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22564259
ABSTRACT
A novel and expedient histological tetrachrome technique was developed and applied to whole-body sections of the clam Ruditapes decussatus (L. 1758). The technique involves fixation in Carnoy's fluid followed by immediate embedding in paraffin with staining with a combination of Alcian Blue, Periodic Acid-Schiff's, Haematoxylin and Picric Acid. Fixation and staining was perfect for all tissues and resolved good identification of Perkinsus sp. infection and high structural detail. Among the surveyed fixatives, Bouin-Hollande's fluid also provided good results, however, fixation is potentially longer, polysaccharide staining was less intense and fibres appeared to be better preserved by Carnoy's.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Infecções Protozoárias em Animais
/
Coloração e Rotulagem
/
Bivalves
Limite:
Animals
Idioma:
En
Revista:
J Invertebr Pathol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Portugal