Increase of INS-1 cell apoptosis under glucose fluctuation and the involvement of FOXO-SIRT pathway.
Diabetes Res Clin Pract
; 98(1): 132-9, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22579214
ABSTRACT
AIMS:
Variations of blood glucose level have been reported to be more harmful than sustained high glucose, but the effects on pancreatic ß-cells have not yet been clarified. FOXO transcription factors are important for cell fate. We tried to clarify the effect of glucose variability on INS-1 cells, and the potential mechanisms related with FOXO-SIRT pathway.METHODS:
INS-1 cells were exposed to control, SHG (sustained high glucose) or IHG (intermittent high glucose) alternating every 12 h for 5 days.RESULTS:
INS-1 cells in SHG showed lower apoptosis and higher GSIS than IHG. Deacetylated FOXO and binding with SIRT were higher in SHG than IHG. Administration of PI3K inhibitor and/or SIRT inhibitor increased apoptosis and decreased Mn-SOD and Bcl-2 in SHG.CONCLUSIONS:
[corrected] IHG was more harmful to INS-1 cells than SHG. The degree of phosphorylation and acetylation of FOXO transcription factors were different between SHG and IHG, which might be one mechanism of increased INS-1 cell apoptosis in IHG.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Apoptose
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Células Secretoras de Insulina
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Fatores de Transcrição Forkhead
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Sirtuína 1
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Glucose
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Insulina
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Proteínas do Tecido Nervoso
Limite:
Animals
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Humans
Idioma:
En
Revista:
Diabetes Res Clin Pract
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article