Identification of DNA signatures suitable for use in development of real-time PCR assays by whole-genome sequence approaches: use of Streptococcus pyogenes in a pilot study.
J Clin Microbiol
; 50(8): 2770-3, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22593599
ABSTRACT
A stepwise computational approach using three layers of publicly available software was found to effectively identify DNA signatures for Streptococcus pyogenes. PCR testing validated that 9 out of 15 signature-derived primer sets could detect as low as 5 fg of target DNA with high specificity. The selected signature-derived primer sets were successfully evaluated against all 23 clinical isolates. The approach is readily applicable for designing molecular assays for rapid detection and characterization of various pathogenic bacteria.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Streptococcus pyogenes
/
DNA Bacteriano
/
Técnicas Bacteriológicas
/
Primers do DNA
/
Técnicas de Diagnóstico Molecular
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos