Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.
Cell Commun Adhes
; 19(2): 25-37, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22642233
ABSTRACT
Intercellular Ca(2+) wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca(2+) wave propagation between SMCs at the cellular level. Gap junctional communication was assessed by the propagation of intercellular Ca(2+) waves and the transfer of Lucifer Yellow in A7r5 cells, primary rat mesenteric SMCs (pSMCs), and 6B5N cells, a clone of A7r5 cells expressing higher connexin43 (Cx43) to Cx40 ratio. Mechanical stimulation induced an intracellular Ca(2+) wave in pSMC and 6B5N cells that propagated to neighboring cells, whereas Ca(2+) waves in A7r5 cells failed to progress to neighboring cells. We demonstrate that Cx43 forms the functional GJs that are involved in mediating intercellular Ca(2+) waves and that co-expression of Cx40 with Cx43, depending on their expression ratio, may interfere with Cx43 GJ formation, thus altering junctional communication.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Conexina 43
/
Sinalização do Cálcio
/
Miócitos de Músculo Liso
/
Artérias Mesentéricas
Limite:
Animals
Idioma:
En
Revista:
Cell Commun Adhes
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Suíça