Mesoscale DNA structural changes on binding and photoreaction with Ru[(TAP)2PHEHAT]2+.
J Am Chem Soc
; 134(24): 10214-21, 2012 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-22667828
ABSTRACT
We used scanning force microscopy (SFM) to study the binding and excited state reactions of the intercalating photoreagent Ru[(TAP)(2)PHEHAT](2+) (TAP = 1,4,5,8-tetraazaphenanthrene; PHEHAT = 1,10-phenanthrolino[5,6-b]1,4,5,8,9,12-hexaazatriphenylene) with DNA. In the ground state, this ruthenium complex combines a strong intercalative binding mode via the PHEHAT ligand, with TAP-mediated hydrogen bonding capabilities. After visible irradiation, SFM imaging of the photoproducts revealed both the structural implications of photocleavages and photoadduct formation. It is found that the rate of photocleaving is strongly increased when the complex can interact with DNA via hydrogen bonding. We demonstrated that the photoadduct increases DNA rigidity, and that the photo-biadduct can crosslink two separate DNA segments in supercoiled DNA. These mechanical and topological effects might have important implications in future therapeutic applications of this type of compounds.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Rutênio
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DNA
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Substâncias Intercalantes
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Conformação de Ácido Nucleico
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Bélgica