Genetic polymorphisms in detoxification and DNA repair genes and susceptibility to glycidamide-induced DNA damage.
J Toxicol Environ Health A
; 75(13-15): 920-33, 2012.
Article
em En
| MEDLINE
| ID: mdl-22788377
ABSTRACT
Acrylamide (AA) is a probable human carcinogen formed in carbohydrate-rich foodstuffs upon heating. Glycidamide (GA), the AA metabolite formed by epoxidation, is considered the ultimate genotoxic agent. In this study, the in vitro genotoxic potential of AA and GA in human whole blood leukocytes was compared using the alkaline comet assay. Although AA did not induce significant DNA damage in the concentrations tested (up to 1000 µM), GA markedly increased the percentage of tail DNA at concentrations ≥250 µM. Further, this study addressed the role of genetic polymorphisms in key genes involved in metabolism and DNA repair pathways (BER, NER, HRR, and NHEJ) on GA-induced genotoxicity assessed by the alkaline comet assay. The results obtained suggested associations between DNA damage and polymorphisms of BER (MUTYH Gln335His and XRCC1 Gln399Arg) and NER (XPC Ala499Val) genes, either alone or in combination.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Polimorfismo de Nucleotídeo Único
/
DNA Glicosilases
/
Proteínas de Ligação a DNA
/
Compostos de Epóxi
/
Mutagênicos
Limite:
Adult
/
Female
/
Humans
/
Male
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Toxicol Environ Health A
Assunto da revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Portugal