Selective isotope labeling of recombinant proteins in Escherichia coli.
Methods Mol Biol
; 896: 439-48, 2012.
Article
em En
| MEDLINE
| ID: mdl-22821543
ABSTRACT
Selective stable-isotope labeling is a useful technique to study structures of proteins, especially intrinsically disordered proteins, by nuclear magnetic resonance spectroscopy. Here, we describe a simple method for amino acid selective isotope labeling of recombinant proteins in E. coli. This method only requires addition of an excess of unlabeled amino acids and, if necessary, enzyme inhibitors to the culture medium. Its efficiency has been demonstrated even in labeling with glutamine or glutamate that is easily converted to other amino acid types by the metabolic pathways of E. coli.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
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Escherichia coli
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Marcação por Isótopo
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão