Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes.
Appl Environ Microbiol
; 78(20): 7480-2, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22885744
A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Salmonella
/
Variação Genética
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Reação em Cadeia da Polimerase
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Adesinas Bacterianas
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Técnicas Analíticas Microfluídicas
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Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos