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C-Jun terminal kinases play an important role in regulating embryonic survival and eye development in vertebrates.
Huang, X-Q; Huang, Z-X; Li, Z-L; Chen, X-W; Li, X; Tang, X-C; Liu, F-Y; Liu, Y; Chen, L; Han, W-J; Liu, L; Li, L; Wu, K-L; Wu, M-X; Liu, X-L; Luo, L-X; Yu, M-B; Liu, Y-Z; Liu, S-J; Li, D W-C.
Afiliação
  • Huang XQ; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road, Guangzhou, 510060, China.
Curr Mol Med ; 13(1): 228-37, 2013 Jan.
Article em En | MEDLINE | ID: mdl-23116265
ABSTRACT
The c-Jun N-terminal kinases (JNKs) constitute one of the three major types of mitogen-activated protein kinases. Previous studies showed that JNK mediates multiple signaling transduction pathways implicated in cell proliferation, differentiation, inflammation, stress response and apoptosis in mammals. In the present study, we use goldfish as a model system and demonstrate that JNK kinases are necessary to promote embryonic survival and regulate eye development in vertebrates. During goldfish development, JNK1 and JNK2 are expressed at every stage from cleavage to hatching larvae. JNK3 is turned on at the gastrulation stage and then expressed at similar level to that of JNK2. JNK1 activity remains slightly fluctuated during different developmental stages. Inhibition of JNK activity caused massive apoptosis of blastula cells and significant death of goldfish embryos, which are associated with altered expression of the anti-apoptotic regulator, Mcl-1 and the proapoptotic regulator, Bak. These results provide novel information regarding the mechanisms by which JNKs promote embryonic survival. In addition, the embryos that survived inhibition of JNK activity displayed severe phenotype in the eye with clear microphthalmia and lens coloboma. To confirm that the observed phenotype is derived from JNK activity deficiency, we expressed JNK dominant negative mutant (DNM-JNK) in goldfish. Expression of DNM-JNK also caused similar phenotypes with altered expression of pax-6, Sox-2 and ß-crystallin. Together, our results demonstrate that JNKs play important roles in promoting survival of vertebrate embryos and regulating development of vertebrate eye.
Assuntos
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Bases de dados: MEDLINE Assunto principal: Carpa Dourada / MAP Quinase Quinase 4 / Olho Limite: Animals Idioma: En Revista: Curr Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China
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Bases de dados: MEDLINE Assunto principal: Carpa Dourada / MAP Quinase Quinase 4 / Olho Limite: Animals Idioma: En Revista: Curr Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China