Proline-dependent regulation of Clostridium difficile Stickland metabolism.
J Bacteriol
; 195(4): 844-54, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23222730
ABSTRACT
Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, generating ATP and reducing power in the process. Reduction of the electron acceptors proline and glycine requires the d-proline reductase (PR) and the glycine reductase (GR) enzyme complexes, respectively. Addition of proline in the medium increases the level of PR protein but decreases the level of GR. We report the identification of PrdR, a protein that activates transcription of the PR-encoding genes in the presence of proline and negatively regulates the GR-encoding genes. The results suggest that PrdR is a central metabolism regulator that controls preferential utilization of proline and glycine to produce energy via the Stickland reactions.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Prolina
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Regulação Bacteriana da Expressão Gênica
/
Clostridioides difficile
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos