Increase of cyclooxygenase-2 inhibition with celecoxib combined with 5-FU enhances tumor cell apoptosis and antitumor efficacy in a subcutaneous implantation tumor model of human colon cancer.
World J Surg Oncol
; 11: 16, 2013 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-23347845
ABSTRACT
BACKGROUND:
The purpose of this study was to investigate the anti-tumor effect and explore the mechanisms of celecoxib (a selective cyclooxygenase-2 inhibitor) combined with 5-fluorouracil (5-FU) on the treatment of human colorectal cancer in a BALB/C nude mouse subcutaneous xenograft model.METHODS:
Effects of celecoxib combined with 5-FU on the proliferation of xenograft carcinoma induced by HT-29 were investigated. The apoptotic cells were detected by electron microscope and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay. Immunohistochemistry and Western blot were used to estimate the expression of cytochrome C, caspase-3 and caspase-9.RESULTS:
Compared with the control group, treatment groups showed significant inhibition of tumor growth. More apoptotic cells existed after treatment with celecoxib combined with 5-FU. Cytochrome C, caspase-3 and caspase-9 were increased in treated groups, and more obviously in the drug combination group. Cyclooxygenase-2 (COX-2) were decreased after treatment with celecoxib only or combined with 5-FU. And the combined group showed a greater decrease.CONCLUSIONS:
Celecoxib combined with 5-FU could inhibit the growth of tumors in vivo by inducing apoptosis and activation of the cytochrome C dependency apoptosis signal pathway. A decrease of COX-2 and an increase of cytochrome C, caspase-3 and caspase-9 may be involved in this process.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Pirazóis
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Sulfonamidas
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Apoptose
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Neoplasias do Colo
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Inibidores de Ciclo-Oxigenase 2
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Fluoruracila
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Antimetabólitos Antineoplásicos
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
World J Surg Oncol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China