Dung-shen (Codonopsis pilosula) attenuated the cardiac-impaired insulin-like growth factor II receptor pathway on myocardial cells.
Food Chem
; 138(2-3): 1856-67, 2013 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-23411318
ABSTRACT
Previous studies from our lab showed that increase in AngII in H9c2 cells causes elevated IGFII and IGFIIR through MEK and JNK, leading to rise in intracellular calcium, calcineurin activation by PLC-ß3 via Gαq, insertion into mitochondrial membranes of Bad, and apoptosis via caspases 9 and 3. Codonopsis pilosula is traditionally used to lower blood pressure. The purpose of our study is to investigate if C. pilosula attenuates AngII plus Leu(27)-IGFII-induced calcium influx and apoptosis in H9c2 cardiomyoblasts. C. pilosula significantly attenuated AngII induced IGFIIR promoter activity. Leu(27)-IGFII was applied to enhance the AngII effect. C. pilosula also reversed Ca(2+) influx, MOMP and apoptosis increased by AngII plus Leu(27)-IGFII. Molecular markers in IGFIIR apoptotic pathway (IGFIIR, calcineurin, etc.) and IGFIIR-Gαq association were downregulated by C. pilosula. However, p-Bad(Ser136) and Bcl-2 were increased. Therefore, C. pilosula suppresses AngII plus Leu(27)-IGFII-induced IGFII/IGFIIR pathway in myocardial cells.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Receptor IGF Tipo 2
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Codonopsis
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Miócitos Cardíacos
Limite:
Humans
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Taiwan