A concise review of HPLC, LC-MS and LC-MS/MS methods for determination of azithromycin in various biological matrices.
Biomed Chromatogr
; 27(10): 1243-58, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23553351
ABSTRACT
Azithromycin is one of the best selling antibiotics in the world. It belongs to the new macrolide family of azalides. It is derived from erythromycin and it differs from erythromycin in having a 15-membered ring and a methyl substituted nitrogen inserted at the 9a position in the aglycone ring. This structural modification confers favourable microbiological and pharmacokinetic characteristics on azithromycin and greater acid stability compared with erythromycin. It is mainly used to treat respiratory infections, sexually transmitted diseases, cutaneous and soft-tissue infections, etc. This review provides a comprehensive overview of various HPLC, LC-MS and LC-MS/MS methods for quantitation of azithromycin in different biological matrices. In addition, it provides general information on extraction steps, internal standard selection, conditions for chromatographic separation, brief validation data and applicable conclusions for reported methods in a defined pattern.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
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Azitromicina
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Espectrometria de Massas em Tandem
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Fracionamento Químico
Limite:
Animals
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Humans
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Índia