Intercellular trafficking of the nuclear oncoprotein DEK.
Proc Natl Acad Sci U S A
; 110(17): 6847-52, 2013 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-23569252
ABSTRACT
DEK is a biochemically distinct, conserved nonhistone protein that is vital to global heterochromatin integrity. In addition, DEK can be secreted and function as a chemotactic, proinflammatory factor. Here we show that exogenous DEK can penetrate cells, translocate to the nucleus, and there carry out its endogenous nuclear functions. Strikingly, adjacent cells can take up DEK secreted from synovial macrophages. DEK internalization is a heparan sulfate-dependent process, and cellular uptake of DEK into DEK knockdown cells corrects global heterochromatin depletion and DNA repair deficits, the phenotypic aberrations characteristic of these cells. These findings thus unify the extracellular and intracellular activities of DEK, and suggest that this paracrine loop involving DEK plays a role in chromatin biology.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas Cromossômicas não Histona
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Heterocromatina
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Histonas
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Proteínas Oncogênicas
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Comunicação Parácrina
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Reparo do DNA
Limite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos