Retroactivity controls the temporal dynamics of gene transcription.
ACS Synth Biol
; 2(8): 431-41, 2013 Aug 16.
Article
em En
| MEDLINE
| ID: mdl-23654274
ABSTRACT
Just like in many engineering systems, impedance-like effects, called retroactivity, arise at the interconnection of biomolecular circuits, leading to unexpected changes in a circuit's behavior. In this paper, we provide a combined experimental and theoretical study to characterize the effects of retroactivity on the temporal dynamics of a gene transcription module in vivo. The response of the module to an inducer was measured both in isolation and when the module was connected to downstream clients. The connected module, when compared to the isolated module, responded selectively to the introduction of the inducer versus its withdrawal. Specifically, a "sign-sensitive delay" appeared, in which the connected module displayed a time delay in the response to induction and anticipation in the response to de-induction. The extent of these effects can be made larger by increasing the amounts of downstream clients and/or their binding affinity to the output protein of the module. Our experimental results and mathematical formulas make it possible to predict the extent of the change in the dynamic behavior of a module after interconnection. They can be employed to both recover the predictive power of a modular approach to understand systems or as an additional design tool to shape the temporal behavior of gene transcription.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Transcrição Gênica
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Ativação Transcricional
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Regulação da Expressão Gênica
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Retroalimentação Fisiológica
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Redes Reguladoras de Genes
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Modelos Genéticos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
ACS Synth Biol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos