Development of a novel FRET probe for the real-time determination of ceramidase activity.
Chembiochem
; 14(9): 1049-52, 2013 Jun 17.
Article
em En
| MEDLINE
| ID: mdl-23728798
ABSTRACT
Fretful novelty We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real-time.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ceramidas
/
Transferência Ressonante de Energia de Fluorescência
/
Ceramidases
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Revista:
Chembiochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Alemanha