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Silver nanowire-based electrochemical immunoassay for sensing immunoglobulin G with signal amplification using strawberry-like ZnO nanostructures as labels.
Cao, Xia; Liu, Shangwei; Feng, Quanchen; Wang, Ning.
Afiliação
  • Cao X; School of Biochemical and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China. caoxia@ccmu.edu.cn
Biosens Bioelectron ; 49: 256-62, 2013 Nov 15.
Article em En | MEDLINE | ID: mdl-23774162
ABSTRACT
The quick development of nanoscience and nanotechnology has paved the way for ultrasensitive biosensing and analysis. In this work, an ultrasensitive electrochemical immunosensor was developed for the detection of human immunoglobulin G (IgG) by combining with a newly designed trace tag on a disposable immunosensor array. The array was prepared by immobilizing captured antibodies on ultralong Ag nanowires, whilst the trace tag was prepared by loading horseradish peroxidase (HRP)-labeled goat anti-human IgG (HRP-anti-IgG) on thionine (TH)-doped mesoporous ZnO nanostrawberries (MP-ZnO). With a sandwich-type immunoassay format, mainly due to crystalline framework and high surface area of the mesoporous (MP) materials, as well as the superconductivity of silver nanowires, the electrochemical signal was significantly amplified. The linear range of the developed immunosensor is 0.01-200 ng mL(-1) and the detection limit is 4 pg mL(-1) IgG, which make the hierarchically nanostructured composites very promising candidates for the next-generation sandwich-type electrochemical immunoassays.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Prata / Óxido de Zinco / Imunoglobulina G / Imunoensaio / Nanoestruturas Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Prata / Óxido de Zinco / Imunoglobulina G / Imunoensaio / Nanoestruturas Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: China