Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system.
Indian J Med Microbiol
; 31(2): 173-6, 2013.
Article
em En
| MEDLINE
| ID: mdl-23867675
ABSTRACT
Success for maximum isolation of anaerobes depends on maintaining critically low oxygen levels throughout and growth in a reduced medium with exclusion of inhibitory substances. Hence a dual system was used equipped with candle combustion for instant exhaustion of major part of oxygen from a sealed jar, along with acidified steel wool for residual oxygen purging. For inhibitory substances removal, test anaerobes were grown on anaerobic medium layered on buffer charcoal agar bed. After 48 hours incubation average colony sizes were compared with that of growths in conventional Gas-Pak system. Better growths were noted in the innovative system.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Bactérias Anaeróbias
/
Técnicas Bacteriológicas
/
Meios de Cultura
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Indian J Med Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Índia