Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases.
Photochem Photobiol Sci
; 13(2): 231-40, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24096539
ABSTRACT
Numerous proteases are known to be necessary for cancer development and progression including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins. The goal of this research is to develop an Fe/Fe3O4 nanoparticle-based system for clinical diagnostics, which has the potential to measure the activity of cancer-associated proteases in biospecimens. Nanoparticle-based "light switches" for measuring protease activity consist of fluorescent cyanine dyes and porphyrins that are attached to Fe/Fe3O4 nanoparticles via consensus sequences. These consensus sequences can be cleaved in the presence of the correct protease, thus releasing a fluorescent dye from the Fe/Fe3O4 nanoparticle, resulting in highly sensitive (down to 1 × 10(-16) mol l(-1) for 12 proteases), selective, and fast nanoplatforms (required time 60 min).
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
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Espectrometria de Fluorescência
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Nanotecnologia
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Ensaios Enzimáticos
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Nanopartículas de Magnetita
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Neoplasias
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Photochem Photobiol Sci
Assunto da revista:
BIOLOGIA
/
QUIMICA
Ano de publicação:
2014
Tipo de documento:
Article