Mechanical wounding of yeast-like conidium cells of Tremella fuciformis makes them susceptible to Agrobacterium-mediated transformation.

ABSTRACT

We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.