Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection.
J Am Soc Mass Spectrom
; 6(3): 220-1, 1995 Mar.
Article
em En
| MEDLINE
| ID: mdl-24214121
ABSTRACT
For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5×10(-14) mol (0.5 µL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 851 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 µs from a 0.15-µL/min injection of 20-µM ubiquitin consumes 5×10(-18) mol, which produces a spectrum with 231 signal-to-noise ratio and τ;3×10(5) resolving power.
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1
Bases de dados:
MEDLINE
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos