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Developmental potential and kinetics of pig embryos with different cytoplasmic volume.
Li, Juan; Li, Rong; Villemoes, Klaus; Liu, Ying; Purup, Stig; Callesen, Henrik.
Afiliação
  • Li J; College of Animal Science and Technology,Nanjing Agricultural University,210095,Jiangsu Province,Nanjing,Wei Gang No. 1,China.
  • Li R; Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
  • Villemoes K; Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
  • Liu Y; Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
  • Purup S; Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
  • Callesen H; Department of Animal Science,Aarhus University,DK-8830 Tjele,Denmark.
Zygote ; 23(2): 277-87, 2015 Apr.
Article em En | MEDLINE | ID: mdl-24229766
The effects of cytoplasmic volumes on development and developmental kinetics of in vitro produced porcine embryos were investigated. During hand-made cloning (HMC), selected cytoplasts were separated into two groups according to their size in relation to the initial oocyte: ~75% or ~50%. Following two fusion steps and activation (day 0), reconstructed embryos were cultured in vitro for 6 days. Cleavage rates on day 2 as well as blastocyst rates and cell numbers on day 6 were recorded. Results showed that embryo development was no different for ~50% versus ~75% cytoplasm at first fusion. This result was used in the following experiments, where the effect of varying cytoplasm volume in second fusion to obtain a final cytoplasm volume of ~75% to ~200% was tested. The results showed that the lowest quality was obtained when the final cytoplasm volume was ~75% and the highest quality at ~200% of the original oocyte. Similar results were observed in parthenogenetic (PA) embryos activated with different cytoplasmic volumes. A common pattern for the developmental kinetics of HMC and PA embryos was observed: the smaller group tended to have a longer time for the first two cell cycles, but subsequently a shorter time to form morula and blastocyst. In conclusion, the developmental kinetics of in vitro produced embryos was affected by the cytoplasm volume of the initial oocyte, and this further accounted for the developmental ability of the reconstructed embryos.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Partenogênese / Blastocisto / Citoplasma / Sus scrofa / Técnicas de Transferência Nuclear Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Partenogênese / Blastocisto / Citoplasma / Sus scrofa / Técnicas de Transferência Nuclear Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China