Class I major histocompatibility complex, the trojan horse for secretion of amyloidogenic ß2-microglobulin.
J Biol Chem
; 289(6): 3318-27, 2014 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-24338476
ABSTRACT
To form extracellular aggregates, amyloidogenic proteins bypass the intracellular quality control, which normally targets unfolded/aggregated polypeptides. Human D76N ß2-microglobulin (ß2m) variant is the prototype of unstable and amyloidogenic protein that forms abundant extracellular fibrillar deposits. Here we focus on the role of the class I major histocompatibility complex (MHCI) in the intracellular stabilization of D76N ß2m. Using biophysical and structural approaches, we show that the MHCI containing D76N ß2m (MHCI76) displays stability, dissociation patterns, and crystal structure comparable with those of the MHCI with wild type ß2m. Conversely, limited proteolysis experiments show a reduced protease susceptibility for D76N ß2m within the MHCI76 as compared with the free variant, suggesting that the MHCI has a chaperone-like activity in preventing D76N ß2m degradation within the cell. Accordingly, D76N ß2m is normally assembled in the MHCI and circulates as free plasma species in a transgenic mouse model.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Antígenos de Histocompatibilidade Classe I
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Microglobulina beta-2
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Mutação de Sentido Incorreto
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Amiloide
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Itália