Development of a method for the determination of cefovecin in plasma by HPLC.
Biomed Chromatogr
; 28(8): 1126-30, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24420236
ABSTRACT
A simple high-performance liquid chromatography method for the determination of cefovecin in small volume plasma has been developed. Following solid-phase extraction using Oasis HLB cartridges, samples were separated by reverse-phase high-performance liquid chromatography on an XBridge C8 (3.5 µm) 4.6 × 250 mm column and quantified using ultraviolet detection at 280 nm. The mobile phase was a mixture of 10 mm ammonium acetate (pH 3.5) and acetonitrile (8911), with a flow rate of 0.85 mL/min. The standard curve ranged from 0.1 to 200 µg/mL. Intra- and Inter-assay variability for cefovecin was <10%, and the average recovery was >90%. The lower limit of quantitation was 0.1 µg/mL. This method was successfully applied to the analysis of cefovecin samples at our institution. This is also the first fully validated method with an internal standard that does not use mass spectrometry.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cefalosporinas
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos