Metal stopping reagents facilitate discontinuous activity assays of the de novo purine biosynthesis enzyme PurE.

Sullivan, Kelly L; Huma, Loredana C; Mullins, Elwood A; Johnson, Michael E; Kappock, T Joseph

Sullivan, Kelly L; Huma, Loredana C; Mullins, Elwood A; Johnson, Michael E; Kappock, T Joseph.

Afiliação

Sullivan KL; Department of Biochemistry, Purdue University, West Lafayette, IN 47907-2063, USA.
Huma LC; Center for Pharmaceutical Biotechnology and Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60607-7173, USA.
Mullins EA; Department of Biochemistry, Purdue University, West Lafayette, IN 47907-2063, USA; Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130-4899, USA.
Johnson ME; Center for Pharmaceutical Biotechnology and Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60607-7173, USA.
Kappock TJ; Department of Biochemistry, Purdue University, West Lafayette, IN 47907-2063, USA. Electronic address: kappock@purdue.edu.

Anal Biochem ; 452: 43-5, 2014 May 01.

Article em En | MEDLINE | ID: mdl-24525042

Assuntos

Ensaios Enzimáticos/métodos; Isomerases/química; Isomerases/metabolismo; Purinas/biossíntese; Sulfato de Zinco/química; Ensaios de Triagem em Larga Escala; Ribonucleotídeos/metabolismo

Palavras-chave

Aminoimidazole; Chelation; Purine biosynthesis; Substrate depletion

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Purinas / Sulfato de Zinco / Ensaios Enzimáticos / Isomerases Idioma: En Revista: Anal Biochem Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos

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