Strigolactone-regulated proteins revealed by iTRAQ-based quantitative proteomics in Arabidopsis.
J Proteome Res
; 13(3): 1359-72, 2014 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-24559214
ABSTRACT
Strigolactones (SLs) are a new class of plant hormones. In addition to acting as a key inhibitor of shoot branching, SLs stimulate seed germination of root parasitic plants and promote hyphal branching and root colonization of symbiotic arbuscular mycorrhizal fungi. They also regulate many other aspects of plant growth and development. At the transcription level, SL-regulated genes have been reported. However, nothing is known about the proteome regulated by this new class of plant hormones. A quantitative proteomics approach using an isobaric chemical labeling reagent, iTRAQ, to identify the proteome regulated by SLs in Arabidopsis seedlings is presented. It was found that SLs regulate the expression of about three dozen proteins that have not been previously assigned to SL pathways. These findings provide a new tool to investigate the molecular mechanism of action of SLs.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Reguladores de Crescimento de Plantas
/
Arabidopsis
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Regulação da Expressão Gênica de Plantas
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Proteínas de Arabidopsis
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Plântula
/
Lactonas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Proteome Res
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos