Distinct B-cell populations contribute to vaccine antigen-specific antibody production in a transgenic mouse model.
Immunology
; 142(4): 624-35, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24645831
ABSTRACT
The generation of memory B cells by vaccination plays a critical role in maintaining antigen-specific antibodies and producing antibody responses upon re-exposure to a pathogen. B-cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus-like particle (VLP) vaccine in a transgenic mouse model that would identify germinal centre-derived memory B cells with the expression of yellow fluorescent protein (YFP(+) cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP(+) cells although vaccine antigen-specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/PR8). In addition, CD43(+) B220(-) populations with low YFP(+) cells mainly contributed to the production of vaccine antigen-specific IgG isotype-switched antibodies whereas CD43(-) B220(+) populations with high YFP(+) cells were able to produce vaccine antigen-specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP(+) cells in the B220(-) populations of spleen and bone marrow cells. These results suggest that CD43(+) B220(-) B cells generated by vaccination are important for producing influenza vaccine antigen-specific antibodies and conferring protection.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Vacinas contra Influenza
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Subpopulações de Linfócitos B
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Memória Imunológica
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Antígenos Virais
Limite:
Animals
Idioma:
En
Revista:
Immunology
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos