Comparative analysis of internal ribosomal entry sites as molecular tools for bicistronic expression.
J Biotechnol
; 181: 31-4, 2014 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-24709397
ABSTRACT
Internal ribosomal entry sites (IRESes) are sequences that drive cap-independent translation. They are found in some viral and cellular transcripts and they have been extensively used in both basic and applied research for the translation of two or more polypeptides from a single mRNA molecule in eukaryotic cells. Although the most widely used IRES comes from the encephalomyocarditis virus (EMCV), several other viral and cellular IRES elements have been identified and successfully used, including those of the human VCIP gene and the mouse Gtx gene. In this report we have compared the EMCV IRES with the VCIP and the Gtx IRESes, and we provide evidence that by using the EMCV IRES much higher levels of second cistron expression can be achieved.
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Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Biossíntese de Proteínas
/
Vírus da Encefalomiocardite
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Grécia