miR-10a and miR-10b target the 3'-untranslated region of TBX5 to repress its expression.
Pediatr Cardiol
; 35(6): 1072-9, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24714979
ABSTRACT
As a well-known transcription factor, TBX5 is involved in embryonic cardiac development. Although TBX5 functions in a dose-dependent manner, the posttranscriptional regulation of human TBX5 is poorly understood. Thus, this study aimed to identify microRNAs that modulate TBX5 expression. Luciferase assays were used to screen miRNAs predicted to modulate TBX5 expression. Using quantitative reverse transcriptase-polymerase chain reaction and Western blot analysis, the authors found that miR-10a and miR-10b significantly repressed TBX5 expression and decreased TBX5 protein levels by targeting the TBX5 3'-untranslated region. In addition, miR-10a and miR-10b expression levels were respectively 2.77 and 3.51 times higher in the heart tissues of congenital heart disease patients than in healthy control subjects, suggesting that they are potential diagnostic biomarkers. In conclusion, the study results indicate that miR-10a and miR-10b inhibit TBX5 expression at the level of translation. Higher levels of miR-10a and miR-10b expression are associated with a higher risk of congenital heart defects.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas com Domínio T
/
MicroRNAs
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Cardiopatias Congênitas
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
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Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Pediatr Cardiol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China