Effect of novobiocin on the viability of human gingival fibroblasts (HGF-1).
BMC Pharmacol Toxicol
; 15: 25, 2014 May 01.
Article
em En
| MEDLINE
| ID: mdl-24887242
ABSTRACT
BACKGROUND:
Novobiocin is a coumarin antibiotic, which affects also eukaryotic cells inhibiting activity of Heat shock protein 90 (Hsp90). The Hsp90 represents a molecular chaperone critical for stabilization and activation of many proteins, particularly oncoproteins that drive cancer progression. Currently, Hsp90 inhibitors focus a significant attention since they form a potentially new class of drugs in therapy of cancer. However, in the process of tumorigenesis a significant role is played also by the microenvironment of the tumour, and, in particular, by cancer-associated fibroblasts (CAFs). This study aimed at examination of the effect played by novobiocin on viability of human gingival fibroblasts (HGF-1).METHODS:
The studies were conducted using 24 h cultures of human gingival fibroblasts - HGF-1 (CRL-2014) in Chamber Slides, in presence of 0.1, 0.5, 1.0, 2.5 or 5.0 mM novobiocin. Cell viability was evaluated using fluorescence test, ATP assay and LDH release.RESULTS:
Viability of HGF-1 was drastically reduced after 5 hour treatment with novobiocin in concentrations of 1 mM or higher. In turn, the percentage of LDH-releasing cells after 5 h did not differ from control value although it significantly increased after 10 h incubation with 1 mM and continued to increase till the 20th hour.CONCLUSIONS:
The obtained data indicate that novobiocin may induce death of human gingival fibroblasts. Therefore, application of the Hsp90 inhibitor in neoplastic therapy seems controversial on one hand novobiocin reduces tumour-associated CAFs but, on the other, it may induce a significant destruction of periodontium.
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Bases de dados:
MEDLINE
Assunto principal:
Novobiocina
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Proteínas de Choque Térmico HSP90
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Fibroblastos
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Antibacterianos
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
BMC Pharmacol Toxicol
Ano de publicação:
2014
Tipo de documento:
Article