An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos.
Bioprocess Biosyst Eng
; 38(2): 307-13, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25119301
ABSTRACT
An electrochemical immunosensor based on interdigitated array microelectrodes (IDAMs) was developed for sensitive, specific and rapid detection of chlorpyrifos. Anti-chlorpyrifos monoclonal antibodies were orientedly immobilized onto the gold microelectrode surface through protein A. Chlorpyrifos were then captured by the immobilized antibody, resulting in an impedance change in the IDAMs surface. Electrochemical impedance spectroscopy was used in conjunction with the fabricated sensor to detect chlorpyrifos. Under optimum conditions, the impedance value change of chlorpyrifos was proportional to its concentrations in the range of 10(0)-10(5) ng/mL. The detection limit was found to be 0.014 ng/mL for chlorpyrifos. The proposed chlorpyrifos immunosensor could be used as a screening method in pesticide determination for the analysis of environmental, agricultural and pharmaceutical samples due to its rapidity, sensitivity and low cost.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Condutometria
/
Análise em Microsséries
/
Clorpirifos
/
Microeletrodos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2015
Tipo de documento:
Article