Candida albicans adherence to denture base material: chemical disinfection and the effect of acquired salivary pellicle formation.
J Prosthodont
; 24(3): 200-6, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25142962
ABSTRACT
PURPOSE:
The aim of this study was to evaluate the effect of 1% sodium hypochlorite (H1%) and 4% chlorhexidine gluconate (CG4%) on the adhesion of Candida albicans to denture base acrylic resins, as well as to verify the effect of the acquired salivary pellicle (ASP) formation on this process. MATERIALS ANDMETHODS:
A total of 300 acrylic specimens were immersed in distilled water (control) (n = 100), H1% (n = 100), or CG4% (n = 100) for 30 days. Twenty specimens were used in each experimental period (0, 1, 7, 15, 30 days). At the end of disinfection testing periods, 10 specimens of each group were exposed to human whole saliva to simulate ASP formation, and then all specimens were incubated with C. albicans ATTC 90028. Microorganism adhesion was analyzed by fluorescence microscopy, after staining with Acridine orange.RESULTS:
In the 30(th) disinfection cycle in relation to baseline, the H1% or CG4%, without ASP formation, reduced the C. albicans adhesion by approximately 80%; however, with ASP, this reduction after disinfection with H1% was higher (88%). The presence of ASP resulted in higher reduction of adhered fungal cells in comparison to resin without ASP, at the 1(st) H1% or CG4% disinfection cycle, as well as at 30(th) H1% disinfection cycles.CONCLUSIONS:
Our results suggest that the presence of saliva might influence the adhesion of C. albicans and improve the effectiveness of methods to reduce fungal adhesion.Palavras-chave
Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Saliva
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Candida albicans
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Adesividade
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Desinfetantes de Equipamento Odontológico
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Película Dentária
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Bases de Dentadura
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Higienizadores de Dentadura
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Carga Bacteriana
Limite:
Humans
Idioma:
En
Revista:
J Prosthodont
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
República Dominicana