An evaluation of genotoxicity and cytotoxicity of melamine in combination with cyanuric acid at three mass ratios.
Biomed Environ Sci
; 27(8): 641-5, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-25189612
ABSTRACT
Melamine in combination with cyanuric acid has been considered to be more toxic than either melamine or cyanuric acid alone. The objective of this study was designed to evaluate the combined genotoxicity and cytotoxicity of melamine (M) and cyanuric acid (C) at three mass ratios (11, 12, 21). MC (11), MC (12), and MC (21) were evaluated for their potential genotoxic risk, at gene level by Ames test, and at chromosomal level by micronucleus test. In order to evaluate cytotoxicity in HEK-293 cells, the MTT assay was included. Western blot was also employed to investigate the renal injury molecule-1 (Kim-1) expression in HEK-293 cells exposed to MC. Neither genotoxicity at gene level nor at chromosomal level was observed for MC (11), MC (12), and MC (21). Based on MTT assay, three ratios of MC at 82.5 and 165 µg/mL slightly inhibited viability of HEK-293 cells (P<0.05). MC (11) at 41.25 and 82.50 µg/mL could elevate the Kim-1 expression in HEK-293 cells.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Receptores Virais
/
Triazinas
/
Glicoproteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
Biomed Environ Sci
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China