Enhancement in production of recombinant two-chain Insulin Glargine by over-expression of Kex2 protease in Pichia pastoris.
Appl Microbiol Biotechnol
; 99(1): 327-36, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25239036
ABSTRACT
Glargine is an analog of Insulin currently being produced by recombinant DNA technology using two different hosts namely Escherichia coli and Pichia pastoris. Production from E. coli involves the steps of extraction of inclusion bodies by cell lysis, refolding, proteolytic cleavage and purification. In P. pastoris, a single-chain precursor with appropriate disulfide bonding is secreted to the medium. Downstream processing currently involves use of trypsin which converts the precursor into two-chain final product. The use of trypsin in the process generates additional impurities due to presence of Lys and Arg residues in the Glargine molecule. In this study, we describe an alternate approach involving over-expression of endogenous Kex2 proprotein convertase, taking advantage of dibasic amino acid sequence (Arg-Arg) at the end of B-chain of Glargine. KEX2 gene over-expression in Pichia was accomplished by using promoters of varying strengths to ensure production of greater levels of fully functional two-chain Glargine product, confirmed by HPLC and mass analysis. In conclusion, this new production process involving Kex2 protease over-expression improves the downstream process efficiency, reduces the levels of impurities generated and decreases the use of raw materials.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
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Pichia
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Biotecnologia
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Expressão Gênica
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Tecnologia Farmacêutica
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Insulina de Ação Prolongada
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2015
Tipo de documento:
Article