Development of an online sulfur-oxidizing bacteria biosensor for the monitoring of water toxicity.
Appl Biochem Biotechnol
; 174(7): 2585-93, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25253265
ABSTRACT
A toxicity monitoring system based on the metabolic properties of sulfur-oxidizing bacteria (SOB) in continuous and fed-batch modes has been applied for the detection of nitrite (NO2 (-)-N). In this study, the effects of different concentrations of NO2 (-)-N (0.1 to 5 mg/L) on the SOB bioreactors were tested. We found that 5 mg/L NO2 (-)-N was very toxic to the SOB bioreactors in both continuous (R1) and fed-batch (R2) modes, showing complete inhibition of SOB activity within 2 h of operation. R1 and R2 were operated in different ways; however, the EC inhibition and recovery patterns were very similar. The EC rate increased with an increasing NO2 (-)-N concentration in both continuous and fed-batch modes. The addition of 5 mg/L NO2 (-)-N in continuous mode decreased the average EC rate by 14.38 ± 2.1 µS/cm/min; while in fed-batch mode, the EC rate decreased by 23 µS/cm/min. Although the toxicity monitoring system could detect 0.5-5 mg/L NO2 (-)-N, it could not detect 0.1 mg/L NO2 (-)-N in either continuous or fed-batch operation. Thus, the SOB biosensor method presented is useful to detect toxic agents such as NO2 (-)-N within a few minutes or hours.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Enxofre
/
Bactérias
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Poluição Química da Água
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Técnicas Biossensoriais
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Monitoramento Ambiental
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Coréia do Sul