Streptomyces vulcanius sp. nov., a novel actinomycete isolated from volcanic sediment.

ABSTRACT

A novel actinomycete, designated strain NEAU-C3(T), was isolated from volcanic sediment collected from Longwan, Jilin province, north China and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Streptomyces. EzTaxon-e analysis of the 16S rRNA gene sequence indicated strain NEAU-C3(T) to be most closely related to Streptomyces hygroscopicus subsp. ossamyceticus JCM 4965(T) (97.7 % sequence similarity) and Streptomyces torulosus JCM 4872(T) (97.7 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-C3(T) belongs within the genus Streptomyces and forms a separate subclade, an association that was supported by a bootstrap value of 72 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The DNA-DNA hybridization values between strain NEAU-C3(T) and the two most closely related type strains were low enough to justify the assignment of the strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain NEAU-C3(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces vulcanius sp. nov. is proposed. The type strain is NEAU-C3(T) (=CGMCC 4.7177(T)=DSM 42139(T)).