Identification of a Mg2+-sensitive ORF in the 5'-leader of TRPM7 magnesium channel mRNA.
Nucleic Acids Res
; 42(20): 12779-88, 2014 Nov 10.
Article
em En
| MEDLINE
| ID: mdl-25326319
ABSTRACT
TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg2+. Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. The study provides the first example of magnesium channel synthesis being controlled by Mg2+ in higher eukaryotes.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Regulação da Expressão Gênica
/
Fases de Leitura Aberta
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Regiões 5' não Traduzidas
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Canais de Cátion TRPM
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Magnésio
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos