Exosome-associated hepatitis C virus in cell cultures and patient plasma.
Biochem Biophys Res Commun
; 455(3-4): 218-22, 2014 Dec 12.
Article
em En
| MEDLINE
| ID: mdl-25449270
ABSTRACT
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-cell contact. Here we report that HCV is associated with exosomes. Using highly purified exosomes and transmission electron microscopic imaging, we demonstrated that HCV occurred in both exosome-free and exosome-associated forms. Exosome-associated HCV was infectious and resistant to neutralization by an anti-HCV neutralizing antibody. There were more exosome-associated HCV than exosome-free HCV detected in the plasma of HCV-infected patients. These results suggest exosome-associated HCV as an alternative form for HCV infection and transmission.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Hepacivirus
/
Anticorpos Anti-Hepatite C
/
Exossomos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos