Feasibility study of an active wound dressing based on hollow fiber membranes in a porcine wound model.
Burns
; 41(4): 778-88, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25451146
ABSTRACT
INVESTIGATION A novel active wound dressing (AWD) concept based on a microporous hollow fiber membrane network was investigated in an animal model. It provides a local solution-perfused environment for regenerative cell nutrition, wound irrigation, debris removal, electrolyte balancing, pH regulation, and topical antibiosis. The device is capable of supplying soluble factors, as tested experimentally for the recombinant human growth and differentiation factor-5 (rhGDF-5). METHODS:
Following in vitro studies for rhGDF-5 using primary human keratinocytes and dermal fibroblasts, we employed a porcine partial thickness wound model with five distinct wounds on each back of n=8 pigs. Four wound groups were perfused differently over 9 days and compared with a negative control wound without perfusion (1) 1% trehalose solution, pH 5.5; (2) rhGDF-5 (150 ng/ml) in 1% trehalose solution, pH 5.5; (3) nutrition solution; and (4) rhGDF-5 (150 ng/ml) in nutrition solution with 1% trehalose, pH 5.5.RESULTS:
Promoted wound healing was observed within group 1 and more pronounced within group 2. Groups 3 and 4, with nutrition solution, showed significant adverse effects on wound healing (p<0.05).CONCLUSIONS:
The investigated AWD concept appears to be an interesting therapeutic tool to study further wound healing support. Additionally, topical application of rhGDF-5 could be promising.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Trealose
/
Cicatrização
/
Queimaduras
/
Queratinócitos
/
Acetato de Sódio
/
Fator 5 de Diferenciação de Crescimento
/
Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Burns
Assunto da revista:
TRAUMATOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Alemanha