Magnetic resonance imaging of stem cell apoptosis in arthritic joints with a caspase activatable contrast agent.
ACS Nano
; 9(2): 1150-60, 2015 Feb 24.
Article
em En
| MEDLINE
| ID: mdl-25597243
ABSTRACT
About 43 million individuals in the U.S. encounter cartilage injuries due to trauma or osteoarthritis, leading to joint pain and functional disability. Matrix-associated stem cell implants (MASI) represent a promising approach for repair of cartilage defects. However, limited survival of MASI creates a significant bottleneck for successful cartilage regeneration outcomes and functional reconstitution. We report an approach for noninvasive detection of stem cell apoptosis with magnetic resonance imaging (MRI), based on a caspase-3-sensitive nanoaggregation MRI probe (C-SNAM). C-SNAM self-assembles into nanoparticles after hydrolysis by caspase-3, leading to 90% amplification of (1)H MR signal and prolonged in vivo retention. Following intra-articular injection, C-SNAM causes significant MR signal enhancement in apoptotic MASI compared to viable MASI. Our results indicate that C-SNAM functions as an imaging probe for stem cell apoptosis in MASI. This concept could be applied to a broad range of cell transplants and target sites.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Artrite
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Células-Tronco
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Imageamento por Ressonância Magnética
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Apoptose
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Meios de Contraste
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Caspase 3
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Articulações
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
ACS Nano
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos