Photo-crosslinkable hydrogel-based 3D microfluidic culture device.
Electrophoresis
; 36(7-8): 994-1001, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25641332
ABSTRACT
We developed the photo-crosslinkable hydrogel-based 3D microfluidic device to culture neural stem cells (NSCs) and tumors. The photo-crosslinkable gelatin methacrylate (GelMA) polymer was used as a physical barrier in the microfluidic device and collagen type I gel was employed to culture NSCs in a 3D manner. We demonstrated that the pore size was inversely proportional to concentrations of GelMA hydrogels, showing the pore sizes of 5 and 25 w/v% GelMA hydrogels were 34 and 4 µm, respectively. It also revealed that the morphology of pores in 5 w/v% GelMA hydrogels was elliptical shape, whereas we observed circular-shaped pores in 25 w/v% GelMA hydrogels. To culture NSCs and tumors in the 3D microfluidic device, we investigated the molecular diffusion properties across GelMA hydrogels, indicating that 25 w/v% GelMA hydrogels inhibited the molecular diffusion for 6 days in the 3D microfluidic device. In contrast, the chemicals were diffused in 5 w/v% GelMA hydrogels. Finally, we cultured NSCs and tumors in the hydrogel-based 3D microfluidic device, showing that 53-75% NSCs differentiated into neurons, while tumors were cultured in the collagen gels. Therefore, this photo-crosslinkable hydrogel-based 3D microfluidic culture device could be a potentially powerful tool for regenerative tissue engineering applications.
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Bases de dados:
MEDLINE
Assunto principal:
Hidrogéis
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Técnicas de Cultura de Tecidos
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Dispositivos Lab-On-A-Chip
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Células-Tronco Neurais
Limite:
Humans
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2015
Tipo de documento:
Article