An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level.
Phys Chem Chem Phys
; 17(33): 21337-42, 2015 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-25712032
ABSTRACT
In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500-1700 cm(-1)). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood samples.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Proteínas
/
Espectroscopia de Infravermelho com Transformada de Fourier
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Phys Chem Chem Phys
Assunto da revista:
BIOFISICA
/
QUIMICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália